Authors: Juan Carlos Stockert, Alfonso Blázquez-Castro

Fluorescence Microscopy in Life Sciences

eBook: US $129 Special Offer (PDF + Printed Copy): US $220
Printed Copy: US $155
Library License: US $516
ISBN: 978-1-68108-519-7 (Print)
ISBN: 978-1-68108-518-0 (Online)
Year of Publication: 2017
DOI: 10.2174/97816810851801170101

Introduction

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

  • - Operation of fluorescence microscopy instruments as well as the different options available today for the scientist, from the classical to the most recent approaches
  • - The wide range of biological detection possibilities that fluorescence microscopy offers in molecular biology, cell biology, histology and histopathology
  • - Fluorescent chemical compounds
  • - Breakthroughs in this field, such as non-linear microscopies and super-resolution techniques

Fluorescence Microscopy In Life Sciences is intended as a detailed guide for professionals, researchers and students (including graduates Ph.D. candidates) in life sciences, with special emphasis in the biomedical field. Researchers working in allied disciplines such as pharmacology, veterinary sciences and microbiology will also benefit from the information presented in this handbook.

Foreword

- Pp. i-ii (2)
Richard W. Horobin
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Preface

- Pp. iii-iv (2)
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Dedication

- Pp. v
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Acknowledgements

- Pp. vi
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Introduction

- Pp. 1-21 (21)
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Physical Fundamentals of Luminescence

- Pp. 22-60 (39)
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Dyes and Fluorochromes

- Pp. 61-95 (35)
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Fluorescent Labels

- Pp. 96-134 (39)
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Spectral Analysis

- Pp. 135-158 (24)
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Fluorescence Instrumental and Techniques

- Pp. 159-218 (60)
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General Fluorescence Reactions

- Pp. 219-251 (33)
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Fluorescent Metal Complexes and Ion Indicators

- Pp. 252-282 (31)
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Luminescent Transition Metal Probes

- Pp. 283-335 (53)
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Lipids

- Pp. 336-358 (23)
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Polysaccharides

- Pp. 359-380 (22)
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Nucleic Acids

- Pp. 381-435 (55)
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Proteins

- Pp. 436-461 (26)
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Enzymes

- Pp. 462-480 (19)
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Vital Probes 1. Cell Organelles

- Pp. 481-531 (51)
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Vital Probes 2. Cell Viability and Signaling

- Pp. 532-567 (36)
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Immunofluorescence and FISH

- Pp. 568-605 (38)
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Luminescent Solid-State Markers

- Pp. 606-641 (36)
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Non-linear Optics

- Pp. 642-686 (45)
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Super-Resolution Microscopy

- Pp. 687-714 (28)
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Subject Index

- Pp. 715-731 (17)
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