Authors: Juan Carlos Stockert, Alfonso Blázquez-Castro

Fluorescence Microscopy in Life Sciences

eBook: US $129 Special Offer (PDF + Printed Copy): US $220
Printed Copy: US $155
Library License: US $516
ISBN: 978-1-68108-519-7 (Print)
ISBN: 978-1-68108-518-0 (Online)
Year of Publication: 2017
DOI: 10.2174/97816810851801170101

Introduction

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

  • - Operation of fluorescence microscopy instruments as well as the different options available today for the scientist, from the classical to the most recent approaches
  • - The wide range of biological detection possibilities that fluorescence microscopy offers in molecular biology, cell biology, histology and histopathology
  • - Fluorescent chemical compounds
  • - Breakthroughs in this field, such as non-linear microscopies and super-resolution techniques

Fluorescence Microscopy In Life Sciences is intended as a detailed guide for professionals, researchers and students (including graduates Ph.D. candidates) in life sciences, with special emphasis in the biomedical field. Researchers working in allied disciplines such as pharmacology, veterinary sciences and microbiology will also benefit from the information presented in this handbook.

Foreword

- Pp. i-ii (2)
Richard W. Horobin
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Preface

- Pp. iii-iv (2)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Dedication

- Pp. v
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Acknowledgements

- Pp. vi
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Introduction

- Pp. 1-21 (21)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Physical Fundamentals of Luminescence

- Pp. 22-60 (39)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Dyes and Fluorochromes

- Pp. 61-95 (35)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Fluorescent Labels

- Pp. 96-134 (39)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Spectral Analysis

- Pp. 135-158 (24)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Fluorescence Instrumental and Techniques

- Pp. 159-218 (60)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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General Fluorescence Reactions

- Pp. 219-251 (33)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Fluorescent Metal Complexes and Ion Indicators

- Pp. 252-282 (31)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Luminescent Transition Metal Probes

- Pp. 283-335 (53)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Lipids

- Pp. 336-358 (23)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Polysaccharides

- Pp. 359-380 (22)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Nucleic Acids

- Pp. 381-435 (55)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Proteins

- Pp. 436-461 (26)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Enzymes

- Pp. 462-480 (19)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Vital Probes 1. Cell Organelles

- Pp. 481-531 (51)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Vital Probes 2. Cell Viability and Signaling

- Pp. 532-567 (36)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Immunofluorescence and FISH

- Pp. 568-605 (38)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Luminescent Solid-State Markers

- Pp. 606-641 (36)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Non-linear Optics

- Pp. 642-686 (45)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Super-Resolution Microscopy

- Pp. 687-714 (28)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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Subject Index

- Pp. 715-731 (17)
Juan Carlos Stockert, Alfonso Blazquez-Castro
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