Editor: Kevin R. Smith

Sperm-Mediated Gene Transfer: Concepts and Controversies

eBook: US $59 Special Offer (PDF + Printed Copy): US $136
Printed Copy: US $107
Library License: US $236
ISBN: 978-1-60805-432-9 (Print)
ISBN: 978-1-60805-237-0 (Online)
Year of Publication: 2012
DOI: 10.2174/97816080523701120101

Introduction

Sperm-mediated gene transfer (SMGT) represents a novel set of technologies for animal (or in the future, human) genetic modification using the sperm as a vector, as opposed to more traditional established routes such as fertilized eggs or embryonic stem cells.

Studies of sperm-mediated gene transfer (SMGT) indicate that sperm cells possess the ability to be utilized as carriers of exogenous genetic sequences, offering the potential of a novel cost-effective route for germline genetic modification. The fate of transgenes borne by sperm cells has been inconsistent, and analysis of offspring from SMGT experiments has shown a mixed picture in terms of genomic integration of the transgene, suggesting an episomal mode of inheritance. Various distinct steps in transgene uptake by the sperm cell have been described or proposed, including a model based upon endogenous reverse transcriptase activity. Although mature sperm cells are naturally protected against uptake of foreign nucleic acid molecules, certain environmental conditions, for example at key times within the reproductive tract, may reduce this protection, suggesting that SMGT may occasionally take place in nature. If correct, this carries profound implications for evolution and human genetic health. This e-book brings together theoretical and empirical reviews from experts in SMGT, providing comprehensive coverage of the major trends, developments and controversies in this novel field. This e-book is intended as a reference for professional researchers in the field of animal genetic modification (transgenesis) as well as teachers, scientists and physicians interested in medical genetics in general and gene therapy in particular.

Indexed in: Book Citation Index, Science Edition, Web of Science, BIOSIS Previews, Scopus, Chemical Abstracts, EBSCO.

Contributors

Editor(s):
Kevin R. Smith
Abertay University
UK




Contributor(s):
Pablo Bermejo-Álvarez
Department of Animal Reproduction
INIA, Ctra de la Coruña KM 5.9
Madrid, 28040
Spain


Sebastian Canovas
University of Murcia
Spain


Tiago Collares
Biotechnology Centre
Federal University of Pelotas
Brazil


Odir A. Dellagostin
Biotechnology Centre
Federal University of Pelotas
Brazil


João Carlos Deschamps
Biotechnology Centre
Federal University of Pelotas
Brazil


Vinicius Farias Campos
Biotechnology Centre
Federal University of Pelotas
Brazil


Raúl Fernández-González
Department of Animal Reproduction
INIA, Ctra de la Coruña KM 5.9
Madrid, 28040
Spain


Celia Frutos
Department of Animal Reproduction
INIA, Ctra de la Coruña KM 5.9
Madrid, 28040
Spain


Joaquin Gadea
University of Murcia
Spain


Francisco A. Garcia-Vazquez
University of Murcia
Spain


Nasser Ghanem
Cairo University
Egypt


Michael Gurevich
Tel HaShomer Hospital
Israel


Alfonso Gutiérrez-Adán
Department of Animal Reproduction
INIA, Ctra de la Coruña KM 5.9
Madrid, 28040
Spain


Eliane Harel-Markowitz
Kimron Veterinary Institute
Israel


Michael Hölker
University of Bonn
Germany


Ann Kiessling
Harvard Medical School
Bedford Research Foundation
USA


Ricardo Laguna
Department of Animal Reproduction
INIA, Ctra de la Coruña KM 5.9
Madrid, 28040
Spain


Carlos Frederico Ceccon Lanes
University of Nordland
Norway


Luis F. Marins
Universidade Federal do Rio Grande (FURG)
Brazil


Alberto Miranda
Department of Animal Reproduction
INIA, Ctra de la Coruña KM 5.9
Madrid, 28040
Spain


John Parrington
University of Oxford
UK


Miriam Pérez-Crespo
Department of Animal Reproduction
INIA, Ctra de la Coruña KM 5.9
Madrid, 28040
Spain


Karl Schellander
University of Bonn
Germany


Ilaria Sciamanna
Italian National Health Institute
Viale Regina Elena 299
Rome, 00161
Italy


Fabiana K. Seixas
Biotechnology Centre
Federal University of Pelotas
Brazil


Mordechai Shemesh
Kimron Veterinary Institute
Israel


Laurence Shore
Kimron Veterinary Institute
Israel


Kevin R. Smith
Abertay University
UK


Corrado Spadafora
Italian National Health Institute
Viale Regina Elena 299
Rome, 00161
Italy


Yehuda Stram
Kimron Veterinary Institute
Israel


Dawit Tesfaye
University of Bonn
Germany


Wei Shen
Qingdao Agricultural University
China


Xiaofeng Sun
Qingdao Agricultural University
China


Yidong Niu
Peking University People’s Hospital
China




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