Author: Kuo Ping Chiu

Next-Generation Sequencing and Sequence Data Analysis

eBook: US $49 Special Offer (PDF + Printed Copy): US $131
Printed Copy: US $107
Library License: US $196
ISBN: 978-1-68108-093-2 (Print)
ISBN: 978-1-68108-092-5 (Online)
Year of Publication: 2015
DOI: 10.2174/97816810809251150101

Introduction

Nucleic acid sequencing techniques have enabled researchers to determine the exact order of base pairs - and by extension, the information present - in the genome of living organisms. Consequently, our understanding of this information and its link to genetic expression at molecular and cellular levels has lead to rapid advances in biology, genetics, biotechnology and medicine. Next-Generation Sequencing and Sequence Data Analysis is a brief primer on DNA sequencing techniques and methods used to analyze sequence data. Readers will learn about recent concepts and methods in genomics such as sequence library preparation, cluster generation for PCR technologies, PED sequencing, genome assembly, exome sequencing, transcriptomics and more.

This book serves as a textbook for students undertaking courses in bioinformatics and laboratory methods in applied biology. General readers interested in learning about DNA sequencing techniques may also benefit from the simple format of information presented in the book.

About the Author

- Pp. i
Kuo Ping Chiu
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Foreword

- Pp. ii
Miao-Lin (Merlin) Hu
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Preface

- Pp. iii-iv (2)
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Acknowledgements

- Pp. v
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The Ultimate Frontier of Knowledge: the Mysterious Genomes and the Gene Expression and Regulation in the Upstream of Biological Information Flow

- Pp. 4-16 (13)
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Evolution of DNA Sequencing Technologies

- Pp. 17-24 (8)
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Mechanisms of Next-Generation Sequencing (NGS)

- Pp. 25-37 (13)
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Genome Assembly, the Genomic Era and the Rise of the Omics Era

- Pp. 38-48 (11)
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Laboratory Setup and Fundamental Works

- Pp. 50-56 (7)
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Sequencing Libraries and Basic Procedure for Sequencing Library Construction

- Pp. 57-61 (5)
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Paired-End (PE), Mate Pair (MP) and Paired-End Ditag (PED) Sequencing

- Pp. 62-76 (15)
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Genome Sequencing and Assembly

- Pp. 77-83 (7)
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Exome Sequencing: Genome Sequencing Focusing on Exonic Regions

- Pp. 84-87 (4)
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Transcriptome Analysis

- Pp. 88-96 (9)
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Single Cell Sequencing (SCS) and Single Cell Transcriptome (SCT) Sequencing

- Pp. 97-104 (8)
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ChIP-TFBS Analysis

- Pp. 105-111 (7)
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ChIP-EM Libraries

- Pp. 112-116 (5)
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MicroRNA Analysis

- Pp. 117-120 (4)
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Application of NGS in the Study of Sequence Diversity in Immune Repertoire

- Pp. 121-126 (6)
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Galaxy Pipeline for Transcriptome Library Analysis

- Pp. 128-146 (19)
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Subject Index

- Pp. 147-148 (2)
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