Author: Kuo Ping Chiu

Next-Generation Sequencing and Sequence Data Analysis

eBook: US $49 Special Offer (PDF + Printed Copy): US $131
Printed Copy: US $107
Library License: US $196
ISBN: 978-1-68108-093-2 (Print)
ISBN: 978-1-68108-092-5 (Online)
Year of Publication: 2015
DOI: 10.2174/97816810809251150101


Nucleic acid sequencing techniques have enabled researchers to determine the exact order of base pairs - and by extension, the information present - in the genome of living organisms. Consequently, our understanding of this information and its link to genetic expression at molecular and cellular levels has lead to rapid advances in biology, genetics, biotechnology and medicine. Next-Generation Sequencing and Sequence Data Analysis is a brief primer on DNA sequencing techniques and methods used to analyze sequence data. Readers will learn about recent concepts and methods in genomics such as sequence library preparation, cluster generation for PCR technologies, PED sequencing, genome assembly, exome sequencing, transcriptomics and more.

This book serves as a textbook for students undertaking courses in bioinformatics and laboratory methods in applied biology. General readers interested in learning about DNA sequencing techniques may also benefit from the simple format of information presented in the book.


I have known Kuo Ping for almost thirty years. He is very fond of science, especially in developing or employing novel technologies for biological investigations. He and I often share ideas and have worked on common scientific interests, including the project to study the early anti-cancer activity of the medicinal fungus Antrodia cinnamomea using Next- Generation Sequencing (NGS) to sequence and compare the transcriptomes of cancer cells treated with or without the extract of A. cinnamomea. This work led to the discovery that this medicinal fungus was able to globally collapse the miRNA system.

It is his never-resting mind that has provided him a motivation to start writing this book some years ago, while he was still working for Genome Institute of Singapore when NGS was starting to take shape. During that time, there was nowhere to find a book to introduce advanced sequencing technologies and the potential applications to either students or laymen. This book evolves from his previous work on paired-end ditag technology and has a strong association with NGS which can be considered as the most fascinating technology of the 21st century. I am glad to see him accomplish this hard task. This book seems to be a good textbook for graduate students and a handbook for researchers who are interested in sequencing and biotechnologies.

It is worth mentioning that Kuo Ping is a scientist with a strong passion for outdoor activities. We did some interesting, and sometimes funny, things together. Back in California, we spent some weekends fishing at Pacifica Pier for perch and crabs, and then barbecued and talked over beer in my backyard. Here in Taiwan, we often tour around his “secret gardens” where he grew trees and raised domestic fish in a pond. The most interesting outdoor experience was a trip to find fossil stones in a wild countryside, where we found forgotten creeks running through the hillside. We tied a very heavy fossil stone with rope and carried it with a bamboo stick across water streams, making loud laughter in the wild. I actually believe that his passion for outdoor activities has contributed to the completion of this useful book.

Miao-Lin (Merlin) Hu, Ph.D.
Honorary Distinguished Professor,
National Chung Hsing University
Department of Food Science and Biotechnology
250 Kuo Kuang Road, Taichung 402