Editors: Chengming Wang, Bernhard Kaltenboeck, Mark D. Freeman

Veterinary PCR Diagnostics

eBook: US $44 Special Offer (PDF + Printed Copy): US $102
Printed Copy: US $80
Library License: US $176
ISBN: 978-1-60805-572-2 (Print)
ISBN: 978-1-60805-348-3 (Online)
Year of Publication: 2012
DOI: 10.2174/97816080534831120101

Introduction

PCR (Polymerase Chain Reaction) technology has become an indispensable component of routine veterinary diagnostics. However, a number of pitfalls and limiting factors affect its sensitivity and specificity of detection. It is imperative that veterinary PCR diagnosticians include such considerations in their work. Extensive experience with PCR technology in both research and diagnostic applications enables researchers to pinpoint these practical limitations, and therefore instruct the user in approaches that avoid these common errors.

This E-book discusses the basic concepts, chemistries, and instrumentation of standard and real-time PCR, and includes present applications and future perspectives for veterinary diagnostics. Critical pitfalls have been highlighted in this text with regard to veterinary PCR diagnostics which include: i) choice of platform technologies for PCR diagnosis; ii) construction of optimized PCR primers and probes to ensure its highest specificity and sensitivity; iii) correct sampling and efficient methods to preserve and release nucleic acids; iv) maximization of PCR amplification efficiency; and v) avoidance and monitoring of contamination in Veterinary PCR Diagnostics. This eBook should be a valuable reference for all veterinary professionals interested in this modern diagnostic tool.

Indexed in: Book Citation Index, Science Edition, Web of Science, Scopus, EBSCO.

Contributors

Editor(s):
Chengming Wang
Yangzhou University
Jiangsu
China


Bernhard Kaltenboeck
Auburn University
AL
USA


Mark D. Freeman
Ross University School of Veterinary Medicine
West Indies




Contributor(s):
Sudhir K. Ahluwalia
Banfield Pet Hospital
10 Traders Way
Salem
MA, 01970
USA


Dawn M. Boothe
Department of Anatomy
Physiology & Pharmacology Auburn University
Auburn
AL
USA


Claudia Calzolari
Department of Veterinary Medical Sciences
University of Bologna
Italy


Mark D. Freeman
Ross University School of Veterinary Medicine
Basseterre St. Kitts
West Indies


Fabio Gentilini
Department of Veterinary Medical Sciences
University of Bologna
Italy


Kirsten Jaegersen
Ross University School of Veterinary Medicine
Basseterre St. Kitts
West Indies


Bernhard Kaltenboeck
Department of Pathobiology
Auburn University
AL
USA


Yihang Li
Department of Veterans Affairs Palo Alto Health Care System
Geriatrics Research, Education and Clinical Center
California
USA


Walter Lilenbaum
Biomedical Institute
Fluminense Federal University
RJ
Brazil


Amanda D. Loftis
Ross University School of Veterinary Medicine
Basseterre St. Kitts
West Indies


Raymaekers Marijke
Clinical Laboratory, Jessa Hospital
Site Virga Jesse
Belgium


Rajesh Nayak
Division of Microbiology
National Center for Toxicological Research
US Food and Drug Administration
Jefferson
AR
USA


Renata F. Rabello
Biomedical Institute
Fluminense Federal University
RJ
Brazil


Will K. Reeves
USAF School of Aerospace Medicine
Wright-Patterson Air Force Base
Ohio
USA


Boakai K. Robertson
Department of Biological Sciences
Alabama State University
Montgomery
AL
USA


Konrad Sachse
Friedrich-Loeffler-Institute
(Federal Research Institute for Animal Health)
Institute of Molecular Pathogenesis
Jena
Germany


Bashar W. Shaheen
Division of Microbiology
National Center for Toxicological Research
US Food and Drug Administration
Jefferson
AR
USA


Rubens C. da Silva Dias
Biomedical Institute
Federal University of State of Rio de Janeiro
Rio de Janeiro
RJ
Brazil


Maria E. Turba
Department of Veterinary Medical Sciences
University of Bologna
Italy


Robert Villafane
Department of Biological Sciences
Alabama State University
Montgomery
AL
USA


Chengming Wang
School of Veterinary Medicine
Yangzhou University
Jiangsu
USA


Hongzhuan Wu
Department of Biological Sciences
Alabama State University
Montgomery
AL
USA




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