Editors: Chengming Wang, Bernhard Kaltenboeck, Mark D. Freeman

Veterinary PCR Diagnostics

eBook: US $44 Special Offer (PDF + Printed Copy): US $102
Printed Copy: US $80
Library License: US $176
ISBN: 978-1-60805-572-2 (Print)
ISBN: 978-1-60805-348-3 (Online)
Year of Publication: 2012
DOI: 10.2174/97816080534831120101

Introduction

PCR (Polymerase Chain Reaction) technology has become an indispensable component of routine veterinary diagnostics. However, a number of pitfalls and limiting factors affect its sensitivity and specificity of detection. It is imperative that veterinary PCR diagnosticians include such considerations in their work. Extensive experience with PCR technology in both research and diagnostic applications enables researchers to pinpoint these practical limitations, and therefore instruct the user in approaches that avoid these common errors.

This E-book discusses the basic concepts, chemistries, and instrumentation of standard and real-time PCR, and includes present applications and future perspectives for veterinary diagnostics. Critical pitfalls have been highlighted in this text with regard to veterinary PCR diagnostics which include: i) choice of platform technologies for PCR diagnosis; ii) construction of optimized PCR primers and probes to ensure its highest specificity and sensitivity; iii) correct sampling and efficient methods to preserve and release nucleic acids; iv) maximization of PCR amplification efficiency; and v) avoidance and monitoring of contamination in Veterinary PCR Diagnostics. This eBook should be a valuable reference for all veterinary professionals interested in this modern diagnostic tool.

Indexed in: Book Citation Index, Science Edition, Web of Science, Scopus, EBSCO.

Foreword

- Pp. i- (1)
Konrad Sachse
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Preface

- Pp. ii
Chengming Wang, Bernhard Kaltenboeck and Mark D. Freeman
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List of Contributors

- Pp. iii-v (3)
Chengming Wang, Bernhard Kaltenboeck and Mark D. Freeman
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Principles of Real-Time PCR

- Pp. 3-17 (15)
Amanda D. Loftis and Will K. Reeves

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Design and Optimization of Real-Time PCR Assays

- Pp. 18-32 (15)
Raymaekers Marijke

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Antimicrobial Resistance in Bacterial Pathogens: Mechanisms and PCRBased Detection Technologies

- Pp. 33-58 (26)
Bashar W. Shaheen, Rajesh Nayak and Dawn M. Boothe

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PCR-Based Diagnosis of Veterinary Bacterial Pathogens

- Pp. 59-79 (21)
Walter Lilenbaum, Renata Fernandes Rabello and Rubens Clayton da Silva Dias

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PCR Detection of Viruses in Veterinary Medicine

- Pp. 80-97 (18)
Yihang Li, Sudhir K. Ahluwalia and Mark D. Freeman

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Quantitative PCR as a Diagnostic Technique in Veterinary Parasitology

- Pp. 98-105 (8)
Hongzhuan Wu, Kirsten Jaegersen, Boakai K. Robertson, Robert Villafane and Chengming Wang

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PCR and Veterinary Cancer Diagnostics

- Pp. 106-135 (30)
Fabio Gentilini, Maria Elena Turba and Claudia Calzolari

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Index

- Pp. 136-137 (2)
Chengming Wang, Bernhard Kaltenboeck and Mark D. Freeman
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