Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach

Authors: Rashid Ali, Ishfaq Ahmad Rather

Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach

ISBN: 978-981-5165-92-0
eISBN: 978-981-5165-91-3 (Online)


This book explores a revolutionary sensing technique called Indicator Displacement Assays (IDAs). It is designed to provide readers with a comprehensive understanding of the conceptual foundation and wide-ranging applications of IDAs. The book aims to fulfill a crucial gap in the existing references on the subject.

The content starts by explaining fundamental concepts, design strategies, and the scope of IDAs. Subsequent chapters elaborate on the intricate molecular recognition of various analytes, offering insights into sensing mechanisms for cationic, anionic, and neutral molecules. The design and construction of colorimetric, fluorescence, and metal complexing IDAs are also thoroughly explored in later chapters, followed by recent extensions of IDAs, including enantioselective indicator displacement assays, intramolecular indicator displacement assays, reaction-based indicator displacement assays, and more. Innovative applications of IDAs, such as sensory arrays and electrochemical sensors, are also discussed in detail, providing a comprehensive understanding of their conceptual foundations and practical implementations.

The book is a primer on IDAs for researchers who want to understand the fundamentals of the technique, as well as postgraduate students aiming to specialize in supramolecular and analytical chemistry.


Chemistry (Supramolecular chemistry, analytical chemistry, material sciences and technology, pharmaceutical chemistry, inorganic chemistry and organic chemistry), pharmaceutical and life sciences researchers; laboratory technicians.


The introduction of diverse indicators to study the molecular recognition (binding/sensing) in various supramolecular receptors opened up a new gateway to mimic nature-based specific and selective host-guest interactions. Taking a step forward from the indicator spacer receptor (ISR) approach involving covalent linkages between receptors and indicators via a spacer, the indicator displacement assay (IDA) offers an innovative and powerful sensing approach for various target analytes in the arena of host-guest chemistry. The intense research in the arena of supramolecular analytical chemistry has led to the development of numerous IDA-based sensors for real-world applications.

The authors have chosen the interesting theme of “Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach” and presented a collection of significant topics covering the design, development, and applications of various types of IDAs and sensory arrays operating through IDA. The corresponding author Dr. Rashid Ali and co-author Ishfaq Ahmad Rather have ample experience in the domain of supramolecular chemistry and have published high-impact research articles in reputed journals. Both authors put their efforts to update the knowledge gained in the field of supramolecular chemistry in general and IDA-based systems in particular. The authors have made an effort to cover a wide range of IDA-based sensors developed from diverse supramolecular receptors like pillarenes, calixarenes, calix[4]pyrroles, cucurbit[n]urils, cyclodextrins, tripodals, boronic acid derivatives, etc. This authored book is a single source of literature collection on the selected topic. In the future plan of action to design, synthesize, and apply IDA-based sensors for real applications, this book will surely help the scientists worldwide already working and also those who want to enter into this emerging arena.

Siddharth Pandey
Indian Institute of Technology
Hauz Khas, New Delhi - 110016