Editors: Sandeep Singh, Dhiraj Kumar

Protocols used in Molecular Biology

eBook: US $59 Special Offer (PDF + Printed Copy): US $100
Printed Copy: US $71
Library License: US $236
ISBN: 978-981-14-3929-2 (Print)
ISBN: 978-981-14-3931-5 (Online)
Year of Publication: 2020
DOI: 10.2174/97898114393151200101
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Introduction

Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Foreword

- Pp. i
Eduardo Sobarzo-Sánchez and Seyed Mohammad Nabavi
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Preface

- Pp. ii
Sandeep Kumar Singh and Dhiraj Kumar
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List of Contributors

- Pp. iii-v (3)
Sandeep Kumar Singh and Dhiraj Kumar
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Isolation of Genomic DNA From Plant Tissues

- Pp. 1-6 (6)
Pallavi Singh

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RNA Isolation Protocol from Cells and Tissues

- Pp. 7-14 (8)
Pallavi Singh

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Analyzing Gene Expression through Real Time PCR while Neo-tissue Regeneration using Developed Tissue Constructs

- Pp. 15-34 (20)
Divakar Singh, Tarun Minocha, Satyavrat Tripathi, Rupika Sinha, Shubhankar Anand, Hareram Birla, Vivek Kumar Pandey, Arun Rawat, Smita Gupta, Sanjeev Kumar Yadav, Pawan Kumar Dubey and Pradeep Srivastava

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A Modified Western Blot Protocol for Enhanced Sensitivity in the Detection of a Tissue Protein

- Pp. 35-43 (9)
Sachchida Nand Rai, Mallikarjuna Rao Gedda, Walia Zahra, Hareram Birla, Saumitra Sen Singh, Payal Singh, Neeraj Tiwari, Rakesh K. Singh and Surya Pratap Singh

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Immunohistochemistry as an Important Technique in Experimental and Clinical Practices

- Pp. 44-59 (16)
Hareram Birla, Sachchida Nand Rai, Saumitra Sen Singh, Walia Zahra, Neeraj Tiwari, Aijaz A. Naik, Anamika Misra, Shikha Bharati and Surya Pratap Singh

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Protocols for the Detection and Proteome Analysis of the Yellow Mosaic Virus Infected Soyabean Leaves

- Pp. 60-66 (7)
Bapatla Kesava Pavan Kumar and Surapathrudu Kanakala

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2D-DIGE A Powerful Tool for Proteome Analysis

- Pp. 67-73 (7)
Sudhir K. Shekhar, Jai Godheja and Dinesh Raj Modi

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Molecular Techniques for Genotyping

- Pp. 74-88 (15)
Shalini Gupta, Somali Sanyal, Suresh Kumar Yadav and Madan Lal Brahma Bhatt

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Sodium Bisulfite Conversion of Human Genome for DNA Methylation Studies

- Pp. 89-96 (8)
Aastha Mishra and Qadar Pasha

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Chromatin Immunoprecipitation (ChIP)

- Pp. 97-113 (17)
Kavyanjali Sharma, Subash Chandra Sonkar and Shakuntala Mahilkar

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Osteosarcoma Cell Culture and Maintenance to Detect the Apoptotic Effect of Some Promising Compounds by Potent Markers viz. DNA

- Pp. 114-119 (6)
Asif Jafri, Juhi Rais, Sudhir Kumar and Md Arshad

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Culture and Maintenance of Human Ovarian Carcinoma Cells for Scrutinizing Anti-cancerous Activities of Various Compounds via Some Potent Molecular Markers

- Pp. 120-125 (6)
Juhi Rais, Asif Jafri, Madhu Tripathi and Md Arshad

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Dictyostelium Discoideum: Live Cell Imaging in Changing Perspective

- Pp. 126-145 (20)
Abhishek Singh

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The Recent Advancement in Rapid Golgi Method and Result Interpretation

- Pp. 146-152 (7)
Surya Prakash Pandey, Mallikarjuna Rao Gedda and Abhishek Pathak

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Molecular Markers for the Evaluation of Clonal Fidelity in Medicinal Plants

- Pp. 153-161 (9)
Arpan Modi and Surapathrudu Kanakala

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Subject Index

- Pp. 162-168 (7)
Sandeep Kumar Singh and Dhiraj Kumar
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