Author: Yusuf Tutar

Essential Techniques for Medical and Life Scientists: A Guide to Contemporary Methods and Current Applications with the Protocols: Part 1

eBook: US $49 Special Offer (PDF + Printed Copy): US $113
Printed Copy: US $89
Library License: US $196
ISBN: 978-1-68108-710-8 (Print)
ISBN: 978-1-68108-709-2 (Online)
Year of Publication: 2018
DOI: 10.2174/97816810870921180101


This handbook covers some primary instruments-based techniques used in modern biological science and medical research programs. Key features of the book include introductory notes for each topic, systematic presentation of relevant methods, troubleshooting guides for practical settings.

Topics covered in the volume include:

  • - Mass spectrometry in proteomics
  • - Structural elucidation of biological macromolecules
  • - Isothermal titration calorimetry
  • - Reverse transcription polymerase chain reaction (RT-PCR)

This book is a simple, useful handbook for students and teachers involved in graduate courses in life sciences and medicine. Readers will learn about the basics of featured techniques, the relevant applications and the established protocols.


Evaluating hypotheses and making judgements on the basis of well supported evidence solve problems in science. Medical and biological data mainly consist of measurement of assay conditions to support the proposed hypothesis. A prediction must test variables and scientist must acquire data from experiments to elicit an explanation or description to the scientific problem. Experimental results of the assay provide direct or indirect evidence to the theory. Therefore, several techniques have been developed to help researchers facilitate experimental design and to elucidate molecular mechanism of biological systems. Some techniques alone may provide unique evidence while still other complement other techniques. For this reason, investigators must be informed about the techniques and know them in detail. It is hard to follow up each technique specially for early career scientists and its applications for experienced researchers. The first volume of this book provides a training platform and application modules of each technique and may help individual investigators to guide research practices.

Prof. Lütfi Tutar
Ahi Evran University