Author: Taseen Gul

Evaluation of Cellular processes by In Vitro Assays

eBook: US $29 Special Offer (PDF + Printed Copy): US $84
Printed Copy: US $69
Library License: US $116
ISBN: 978-1-68108-704-7 (Print)
ISBN: 978-1-68108-703-0 (Online)
Year of Publication: 2018
DOI: 10.2174/97816810870301180101

Introduction

This handbook presents information on different cell culture assays which can be used to perform experimental analysis. Readers are introduced to the basics of in vitro cell cultures followed by a comparative analysis of different experimental protocols designed to detect cellular processes (such as apoptosis, protein-protein interactions, cytotoxicity, gene transfer). Chapters present information on the basics of specific experimental techniques as well as the advantages and disadvantages of the presented methods. Students and scholars who require an understanding of the basic fundamentals of cellular assays.

Foreword

- Pp. i
Khalid Majid Fazili
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Preface

- Pp. ii
Taseen Gul
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Abbreviations

- Pp. iii-iv (2)
Taseen Gul
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Basics of In Vitro Cell Culture

- Pp. 1-19 (19)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq

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Methods of Transfection

- Pp. 20-28 (9)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq

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Apoptosis

- Pp. 29-43 (15)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq

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Cell Cytotoxicity, Viability and Proliferation

- Pp. 44-57 (14)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq

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Reactive Oxygen Species (ROS)

- Pp. 58-67 (10)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq

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Protein-Protein Interactions

- Pp. 68-75 (8)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq

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Protein-Nucleic Acid Interactions

- Pp. 76-84 (9)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq

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Glossary

- Pp. 85-87 (3)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq
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Subject Index

- Pp. 88-94 (7)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq
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