Author: "Taseen Gul"

Evaluation of Cellular processes by In Vitro Assays

Personal Book: US $29 Special Offer (PDF + Printed Copy): US $77
Printed Copy: US $63
Library Book: US $116
ISBN: 978-1-68108-704-7
eISBN: 978-1-68108-703-0 (Online)
DOI: 10.2174/97816810870301180101

Introduction

This handbook presents information on different cell culture assays which can be used to perform experimental analysis. Readers are introduced to the basics of in vitro cell cultures followed by a comparative analysis of different experimental protocols designed to detect cellular processes (such as apoptosis, protein-protein interactions, cytotoxicity, gene transfer). Chapters present information on the basics of specific experimental techniques as well as the advantages and disadvantages of the presented methods. Students and scholars who require an understanding of the basic fundamentals of cellular assays.

Preface

During the course of my doctoral degree, I was very much interested in the methods involved in the understanding effect of drugs in in vitro conditions. The students and scholars often find it confusing to select a particular assay on cell viability, cell proliferation and apoptosis. While teaching at the post-graduate level, I got to know about the problems our students face in understanding the basics of in vitro assays. Keeping in view these facts, I tried to address these concerns by providing a basic introduction about various assays in the form of this book. The book gives comparative analysis of different assays and discusses the advantages and disadvantages related to it. Each experimental protocol is preceded by the information about the principle and the basics of the technique.

I hope my little effort will benefit the students and scholars who are interested to know the basic fundamentals of cellular assays. To make this book better, the criticism and suggestion are most welcomed.

Taseen Gul
Department of Biotechnology,
University of Kashmir,
Hazratbal, Srinagar,
India