Editors: Alane B. Vermelho, Sonia Couri

Methods to Determine Enzymatic Activity

eBook: US $79 Special Offer (PDF + Printed Copy): US $158
Printed Copy: US $119
Library License: US $316
ISBN: 978-1-60805-512-8 (Print)
ISBN: 978-1-60805-300-1 (Online)
Year of Publication: 2013
DOI: 10.2174/97816080530011130101

Introduction

Methods to Determine Enzymatic Activity is a textbook about industrial enzymes. The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text. The main objective of this textbook is to provide readers information focused on the current analysis methods of enzymatic activity at qualitative and quantitative levels. Each chapter is about one specific enzyme and contains information about its substrate and some biochemical properties. The methodologies are presented as an experimental protocol allowing interested readers to reproduce the experimental methods detailed within the textbook. These protocols contain the principle of the technique, materials, methods, and all steps necessary for the determination of enzyme activity and interpretation of results. Each methodology is illustrated with photos and schemes for a better and clear understanding. This book, therefore, uniquely brings modern analysis techniques of industrial enzymes in a single easy to understand volume. This textbook is suitable for undergraduate enzymology courses and advanced industrial biotechnology and microbiology courses.

Indexed in: Book Citation Index, Science Edition, BIOSIS Previews, EBSCO, Ulrich's Periodicals Directory.

Contributors

Editor(s):
Alane B. Vermelho
Biotechnology Center- BIOINOVAR
Bioenergy Biocatalysis and Bioproducts, and
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Sonia Couri
Federal Institute of Education
Science and Technology of Rio de Janeiro
Rio de Janeiro
Brazil




Contributor(s):
Adriana M. Fróes
Department of General Microbiology
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Alane Beatriz Vermelho
Department of General Microbiology
Institute of Microbiology Paulo de Góes
Biotechnology Center- Bioinovar
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Aline Machado de Castro
Biotechnology Division
Research and Development Center
PETROBRAS
Rio de Janeiro
Brazil


Ana Maria Mazotto
Department of General Microbiology
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Andrea M. Salgado
Departament of Biochemical Engineering
Laboratory of Biological Sensors
School of Chemistry
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Antonio Carlos Augusto da Costa
Department of Biochemical Process Technology
Institute of Chemistry
State University of Rio de Janeiro
Rio de Janeiro
Brazil


Ariana F. Melo
Departament of Biochemical Engineering
Laboratory of Biological Sensors
School of Chemistry
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Bernardo Dias Ribeiro
School of Chemistry
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Cristiane Sanchez Farinas
Embrapa Instrumentation
Rio de Janeiro
Brazil


Daniel Paiva
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Edilma Paraguai de Souza
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Giseli Capaci Rodrigues
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Juliana P. Rosa
Department of General Microbiology



Lívia Maria da C. Silva
Departament of Biochemical Engineering
Laboratory of Biological Sensors
School of Chemistry
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Maria Alice Zarur Coelho
Departament of Biochemical Engineering
Laboratory of Biological Sensors
School of Chemistry
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Mônica Caramez Triches Damaso
Embrapa Agroenergy
Brasília
Brazil


Mônica P. Gravina-Oliveira
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Priscilla F. F. Amaral
School of Chemistry
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Rodrigo F. Souza
Department of General Microbiology
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Rodrigo P. Nascimento
School of Chemistry
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Romulo Cardoso Valadão
Federal Rural University of Rio de Janeiro
Institute of Technology
Seropédica
Rio de Janeiro
Brazil


Rosalie R. R. Coelho
Department of General Microbiology
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Sabrina Martins Lage Cedrola
Department of General Microbiology
Institute of Microbiology Paulo de Góes
Federal University of Rio de Janeiro
Rio de Janeiro
Brazil


Selma da Costa Terzi
Embrapa Food Tecnology,
Rio de Janeiro
Brazil


Sonia Couri
Federal Institute of Education
Science and Technology of Rio de Janeiro
Rio de Janeiro
Brazil


Thaís Fabiana Chan Salum
Embrapa Agroenergy
Brasília
Brazil




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