Editors: Alane B. Vermelho, Sonia Couri

Methods to Determine Enzymatic Activity

eBook: US $79 Special Offer (PDF + Printed Copy): US $158
Printed Copy: US $119
Library License: US $316
ISBN: 978-1-60805-512-8 (Print)
ISBN: 978-1-60805-300-1 (Online)
Year of Publication: 2013
DOI: 10.2174/97816080530011130101


Methods to Determine Enzymatic Activity is a textbook about industrial enzymes. The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text. The main objective of this textbook is to provide readers information focused on the current analysis methods of enzymatic activity at qualitative and quantitative levels. Each chapter is about one specific enzyme and contains information about its substrate and some biochemical properties. The methodologies are presented as an experimental protocol allowing interested readers to reproduce the experimental methods detailed within the textbook. These protocols contain the principle of the technique, materials, methods, and all steps necessary for the determination of enzyme activity and interpretation of results. Each methodology is illustrated with photos and schemes for a better and clear understanding. This book, therefore, uniquely brings modern analysis techniques of industrial enzymes in a single easy to understand volume. This textbook is suitable for undergraduate enzymology courses and advanced industrial biotechnology and microbiology courses.

Indexed in: Book Citation Index, Science Edition, BIOSIS Previews, EBSCO, Ulrich's Periodicals Directory.


- Pp. i-ii (2)
Claudiu T. Supuran
Download Free


- Pp. iii-v (3)
Alane Beatriz Vermelho, Sonia Couri
Download Free

List of Contributors

- Pp. vi-viii (3)
Alane Beatriz Vermelho, Sonia Couri
Download Free


- Pp. ix
Alane Beatriz Vermelho, Sonia Couri
Download Free

Methods of Determination of Pectinase Activity

- Pp. 3-26 (24)
Sonia Couri, Selma da Costa Terzi, Bernardo Dias Ribeiro, Antonio Carlos Augusto da Costa, Cristiane Sanchez Farinas
View Abstract

Assays of Peroxidase Activity

- Pp. 27-38 (12)
Bernardo Dias Ribeiro, Maria Alice Zarur Coelho
View Abstract

Methods to Determine Chitinolytic Activity

- Pp. 39-67 (29)
Rosalie R. R. Coelho, Juliana P. Rosa, Rodrigo F. Souza, Adriana M. Fróes
View Abstract

Cellulase Activity Assays: A Practical Guide

- Pp. 68-99 (32)
Cristiane Sanchez Farinas, Mônica Caramez Triches Damaso, Sonia Couri
View Abstract

Methods for Detection of Amylolytic Activities

- Pp. 100-124 (25)
Aline Machado de Castro, Bernardo Dias Ribeiro
View Abstract

Methods to Determine Xylanolytic Activity

- Pp. 125-160 (36)
Rodrigo P. Nascimento, Mônica P. Gravina-Oliveira, Rosalie R. R. Coelho
View Abstract

Assay Methods for Lipase Activity

- Pp. 161-194 (34)
Mônica Caramez Triches Damaso, Thaís Fabiana Chan Salum, Selma Da Costa Terzi, Sonia Couri
View Abstract

Assays of Phenoloxidase Activity

- Pp. 195-207 (13)
Priscilla F. F. Amaral, Bernardo D. Ribeiro
View Abstract

Assay Method for Transglutaminase Activity

- Pp. 208-225 (18)
Mônica Caramez Triches Damaso, Romulo Cardoso Valadão, Sonia Couri, Alane Beatriz Vermelho
View Abstract

Keratinases: Detection Methods

- Pp. 226-261 (36)
Alane Beatriz Vermelho, Ana Maria Mazotto, Sabrina Martins Lage Cedrola
View Abstract

Qualitative and Quantitative to Determine Peptidase Activity

- Pp. 262-280 (19)
Alane Beatriz Vermelho, Edilma Paraguai de Souza, Daniel Paiva, Giseli Capaci Rodrigues
View Abstract

Tannase Activity

- Pp. 281-291 (11)
Ariana F. Melo, Andrea M. Salgado
View Abstract

Urease Activity

- Pp. 292-319 (28)
Lívia Maria da C. Silva, Andrea M. Salgado, Maria Alice Z. Coelho
View Abstract

Subject index

- Pp. 320-322 (3)
Alane Beatriz Vermelho, Sonia Couri
Download Free


.Oxygen: High Enzymatic Reactivity of Reactive Oxygen Species.
.Enzymes Involved in Glycolysis, Fatty Acid and Amino Acid Biosynthesis: Active Site Mechanism and Inhibition.
.Rab GTPases and Membrane Trafficking.