Editors: Alane B. Vermelho, Sonia Couri

Methods to Determine Enzymatic Activity

eBook: US $79 Special Offer (PDF + Printed Copy): US $158
Printed Copy: US $119
Library License: US $316
ISBN: 978-1-60805-512-8 (Print)
ISBN: 978-1-60805-300-1 (Online)
Year of Publication: 2013
DOI: 10.2174/97816080530011130101

Introduction

Methods to Determine Enzymatic Activity is a textbook about industrial enzymes. The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text. The main objective of this textbook is to provide readers information focused on the current analysis methods of enzymatic activity at qualitative and quantitative levels. Each chapter is about one specific enzyme and contains information about its substrate and some biochemical properties. The methodologies are presented as an experimental protocol allowing interested readers to reproduce the experimental methods detailed within the textbook. These protocols contain the principle of the technique, materials, methods, and all steps necessary for the determination of enzyme activity and interpretation of results. Each methodology is illustrated with photos and schemes for a better and clear understanding. This book, therefore, uniquely brings modern analysis techniques of industrial enzymes in a single easy to understand volume. This textbook is suitable for undergraduate enzymology courses and advanced industrial biotechnology and microbiology courses.

Indexed in: Book Citation Index, Science Edition, BIOSIS Previews, EBSCO, Ulrich's Periodicals Directory.

Foreword

- Pp. i-ii (2)
Claudiu T. Supuran
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Preface

- Pp. iii-v (3)
Alane Beatriz Vermelho and Sonia Couri
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List of Contributors

- Pp. vi-viii (3)
Alane Beatriz Vermelho and Sonia Couri
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Acknowledgements

- Pp. ix
Alane Beatriz Vermelho and Sonia Couri
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Methods of Determination of Pectinase Activity

- Pp. 3-26 (24)
Sonia Couri, Selma da Costa Terzi, Bernardo Dias Ribeiro, Antonio Carlos Augusto da Costa and Cristiane Sanchez Farinas

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Assays of Peroxidase Activity

- Pp. 27-38 (12)
Bernardo Dias Ribeiro and Maria Alice Zarur Coelho

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Methods to Determine Chitinolytic Activity

- Pp. 39-67 (29)
Rosalie R. R. Coelho, Juliana P. Rosa, Rodrigo F. Souza and Adriana M. Fróes

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Cellulase Activity Assays: A Practical Guide

- Pp. 68-99 (32)
Cristiane Sanchez Farinas, Mônica Caramez Triches Damaso and Sonia Couri

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Methods for Detection of Amylolytic Activities

- Pp. 100-124 (25)
Aline Machado de Castro and Bernardo Dias Ribeiro

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Methods to Determine Xylanolytic Activity

- Pp. 125-160 (36)
Rodrigo P. Nascimento, Mônica P. Gravina-Oliveira and Rosalie R. R. Coelho

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Assay Methods for Lipase Activity

- Pp. 161-194 (34)
Mônica Caramez Triches Damaso, Thaís Fabiana Chan Salum, Selma Da Costa Terzi and Sonia Couri

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Assays of Phenoloxidase Activity

- Pp. 195-207 (13)
Priscilla F. F. Amaral and Bernardo D. Ribeiro

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Assay Method for Transglutaminase Activity

- Pp. 208-225 (18)
Mônica Caramez Triches Damaso, Romulo Cardoso Valadão, Sonia Couri and Alane Beatriz Vermelho

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Keratinases: Detection Methods

- Pp. 226-261 (36)
Alane Beatriz Vermelho, Ana Maria Mazotto and Sabrina Martins Lage Cedrola

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Qualitative and Quantitative to Determine Peptidase Activity

- Pp. 262-280 (19)
Alane Beatriz Vermelho, Edilma Paraguai de Souza, Daniel Paiva and Giseli Capaci Rodrigues

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Tannase Activity

- Pp. 281-291 (11)
Ariana F. Melo and Andrea M. Salgado

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Urease Activity

- Pp. 292-319 (28)
Lívia Maria da C. Silva, Andrea M. Salgado and Maria Alice Z. Coelho

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Subject index

- Pp. 320-322 (3)
Alane Beatriz Vermelho and Sonia Couri
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