Author: Acharya Balkrishna

Chemical Fingerprints of Medicinal Plants - HPTLC Profiling

eBook: US $49 Special Offer (PDF + Printed Copy): US $88
Printed Copy: US $64
Library License: US $196
ISBN: 978-981-5196-42-9 (Print)
ISBN: 978-981-5196-41-2 (Online)
Year of Publication: 2023
DOI: 10.2174/97898151964121230101


Chemical Fingerprints of Medicinal Plant- HPTLC Profiling gives an overview of the use of high-performance thin-layer chromatography (HPTLC) for profiling medicinal herbs. Starting with an introduction to HPTLC the book guides the reader through HPTLC instrumentation and laboratory application. Additional notes on the stationary HPTLC phases and hyphenating HTPLC with other analytical techniques are also provided. The book concludes with an exhaustive list of HPTLC fingerprints of many herbs that can be used in medicinal preparations. The book outlines a scientific approach to the identification and selection of herbs for preparing traditional medications. By reading this book, learners will be able to 1) understand the basics of HPTLC, 2) learn how to use the instruments for lab analysis and 3) quickly identify chemical fingerprints of important medicinal herbs.

Key Features

  • -   A concise overview of HPTLC and associated techniques
  • -   A Step-by-step guide to performing HPTLC analysis
  • -   Full color illustrations
  • -   An exhaustive list of HPTLC fingerprints for 108 medicinal plants, with botanical names
  • -   An appendix of TLC solvent systems and reagents for different phytochemical compounds and secondary metabolites
  • -   Simple language for easy understanding
  • -   Detailed bibliography

Chemical Fingerprints of Medicinal Plant- HPTLC Profiling is an essential handbook for scholars in analytical chemistry, plant science and traditional medicine who want to understand the application of HPTLC techniques for research and development in phytomedicine.


Scholars in analytical chemistry, plant science and traditional medicine who want to understand the application of HPTLC techniques in phytomedicinal research and development.


Herbs have been used for medicinal purposes since ancient time. A large number of populations in the world rely on herbs and herbal extracts for different ailments. The Indian traditional medicine system – “Ayurveda” also recognizes the importance of the herbs and their authenticity.

In the words of Sage Acharya Charaka,

Tasyāpīyaṃparīkṣā- Idamevaṃprakṛtyevaṃguṇamevaṃprabhāvamsmindeśe

jātamasminnṛtāvevaṃ gṛhītamevaṃnihitamevamupaskṛtamanayā ca mātrayā

CarakaSaṃhitāVimānasthāna 8:87

“Meaning - for selection of herbs Organoleptic, physical and chemical characteristics, habitat, season, method of collection, method of preservation, processing, and dosage should be considered”

With the increasing reliance of the worldwide population on herbs, the selection of the right quality of herbs, identification, and authentication are the need of time. In the modern day, different techniques like taxonomy, biological and chemical fingerprinting are in use to validate the procedure for the selection of the right quality herb.

In the present book, we have developed and compiled the High-Performance Thin Layer Chromatography (HPTLC) fingerprints of some of the commonly used medicinal plants. HPTLC fingerprint provides a scientific approach for the identification and authentication of herbal material as it provides a unique phytochemical distribution pattern which can be seen in the form of bands and can further be used for the purpose of qualitative and quantitative checks.

I am sure that this book will be immensely helpful as a reference book for the scientific community working in this area throughout the nation and world over.

Acharya Balkrishna
University of Patanjali
Patanjali Research Foundation Trust