Editor: A. Mujib

Genome Size and Genetic Homogeneity of Regenerated Plants: Methods and Applications

eBook: US $69 Special Offer (PDF + Printed Copy): US $119
Printed Copy: US $84
Library License: US $276
ISBN: 978-981-5165-56-2 (Print)
ISBN: 978-981-5165-55-5 (Online)
Year of Publication: 2023
DOI: 10.2174/97898151655551230101

Introduction

This reference is a timely compilation of studies of genome size and genetic stability of regenerated plants. It presents 13 book chapters that cover recent advancements in CRISPR/Cas-based genome editing, the use of molecular markers to analyze somaclonal variation in tissue culture, and genetic stability assessment in various plant species, including medicinally valuable plants like Valeriana and Coffea.

The book also highlights the role of flow cytometry in investigating polyploidy and provides valuable insights into genetic fidelity assessment of micropropagated woody plants and orchids. The contributors have shed light on the intra-specific and inter-specific genome and chromosome number variation with reference to gene duplication and DNA sequence loss. Molecular techniques for detecting ploidy levels and genetic homogeneity in regenerated plantlets are also discussed.

Additional highlights of the book include brief guidelines for experimental protocols for flow cytometry and molecular markers, coverage of a wide range of plants, and supporting references. This is an excellent reference for biologists, geneticists, and plant scientists exploring genetic homogeneity and genome size variation in diverse plant groups.

Audience: Researchers in biology, genomics, and plant science, focusing on genome size and genetic homogeneity studies in regenerated plants.

Preface

- Pp. i
A. Mujib
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List of Contributors

- Pp. ii-iv (3)

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Recent Advancements in CRISPR/Cas-based Genome Editing in Plants

- Pp. 1-22 (22)
Anurag Panchal, Tuhin Das, Roshan Kumar Singh, Manoj Prasad*

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Molecular Markers Used in the Analysis of Somaclonal Variation During Plant Tissue Culture

- Pp. 23-42 (20)
Rosalía Núñez-Pastrana, Carlos A. Cruz-Cruz, Marco V. Rodríguez-Deméneghi, Régulo C. Llarena-Hernández, Marco A. Ramírez-Mosqueda*

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Application of Molecular Markers in Revealing Genetic Stability Among In Vitro Regenerants of Different Valeriana Species- a Pharmaceutically Valued Plant

- Pp. 43-59 (17)
Taiba Saeed, Irfan Bashir Ganie, Sabaha Tahseen, Adla Wasi, Zishan Ahmad, Anwar Shahzad*

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Genetic Variation and Genetical Control of Growth and Flowering in Woody Plants: Molecular Techniques

- Pp. 60-72 (13)
Tomohiro Igasaki*

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Somatic Embryogenesis and Genetic Homogeneity Assessment in Coffea - Recent Approaches

- Pp. 73-104 (32)
Manoj Kumar Mishra*, Arun Kumar C. Huded, Pavankumar Jingade

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Cellular and Molecular Tools for the Investigation of Somatic Embryogenesis in Medicago Species

- Pp. 105-124 (20)
Anelia V. Iantcheva*

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Flow Cytometry Analysis of In Vitro Induced Polyploidy in Plants

- Pp. 125-150 (26)
Wudali Narasimha Sudheer, Akshatha Banadka, Praveen Nagella, Jameel M. Al-Khayri*

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Genetic Fidelity Assessment of Micropropagated Woody Plants Through Molecular Analysis

- Pp. 151-179 (29)
Yelda Özden Çiftçi*, Ergun Kaya, Selin Galatali, Damla Ekin Ozkaya, Nil Türkölmez

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Genetic Stability in Micropropagated Orchids: Assessment by Molecular Markers and Flow Cytometry

- Pp. 180-231 (52)
Leimapokpam Tikendra, Abhijit Dey, Manas Ranjan Sahoo, Potshangbam Nongdam*

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Application of Flow Cytometry in Biological Sciences

- Pp. 232-271 (40)
Tahira Jatt*, Majid Ali Maitlo, Sadam Hussain Shaikh, Dessireé Patricia Zerpa-Catanho

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Flow Cytometry-Based Analysis of Tissue Culture-Derived Plants

- Pp. 272-293 (22)
Sara Rahimi, Mojtaba Kordrostami, Ali Akbar Ghasemi-Soloklui, Jameel M. Al-Khayri*

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Molecular Techniques for the Detection of Ploidy Level and Genetic Fidelity of Regenerated Plantlets

- Pp. 294-326 (33)
Gulab Khan Rohela*, Pawan Saini

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Interspecific Genome Size (2C DNA) Variation in Some Ornamental and Medicinal Plants: Is It a Phenomenon of Partial Sequence Amplification or Loss?

- Pp. 327-351 (25)
A. Mujib*, Jyoti Mamgain, Yashika Bansal, Bushra Ejaz

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Subject Index

- Pp. 352-357 (6)
A. Mujib
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