Editors: Kamal Niaz, Muhammad Farrukh Nisar

Series Title: Coronavirus Disease-19 (COVID-19): A Perspective of New Scenario

COVID-19: Epidemiology, Biochemistry, and Diagnostics

Volume 1

eBook: US $79 Special Offer (PDF + Printed Copy): US $142
Printed Copy: US $103
Library License: US $316
ISBN: 978-981-4998-94-9 (Print)
ISBN: 978-981-4998-93-2 (Online)
Year of Publication: 2021
DOI: 10.2174/97898149989321210101

Introduction

COVID-19: Epidemiology, Biochemistry, and Diagnostics explains COVID-19 from multidisciplinary angles such as the evolution of SARS-COV, genetic techniques to study the virus, and diagnostic methodologies widely used in the global COVID-19 pandemic.

The chapters in this book provide the reader with up-to-date literature about research on SARS-CoV-2 through three parts:

I) Evolution and Entry of SARS-CoV-2 into the host

II) Genetic Alteration and Structural Determination of SARS-CoV-2 Proteins

III) Quantitative Analysis of SARS-CoV-2 for research and medical diagnosis

Key Features:

- 15 chapters on SAR-CoV-2 in a multidisciplinary context

- Provides a comprehensive overview of SARS-CoV-2 evolution and genetics

- Provides biochemical information about SARS-CoV-2 proteins and receptor targets (both structural and non-structural proteins)

- Includes an overview of several methods of detecting SARS-CoV-2 virus particles (ELISA, PCR, Neutralizing Antibodies

- Covers some critical diagnostic modalities for COVID-19 diagnosis

- Provides bibliographic references for further reading

Readers will understand the significance of phylogenetic analysis of coronaviruses, along with the pathogenesis of COVID-19 and related diseases such as SARS and MERS. Applications of biochemical technologies such as RT-PCR and CRISPR are also demonstrated in the text. This book is a comprehensive introduction to COVID-19 research for medical researchers, microbiologists and virologists. Students in academic programs in life sciences and medicine will also benefit from the information provided in the book.

Foreword

- Pp. i
Muhammad Sajjad Khan
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Preface

- Pp. ii-iii (2)
Kamal Niaz* and Muhammad Farrukh Nisar*
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List of Contributors

- Pp. iv
Kamal Niaz and Muhammad Farrukh Nisar
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Coronavirus Disease-2019 (COVID- 19) Epidemiology

- Pp. 1-41 (41)
Ihtisham Ulhaq, Abdul Basit, Ijaz Ali, Firasat Hussain, Zahid Ali, Faisal Siddique, Haroon Ahmed, Amjad Islam 3 Aqib and Kashif Rahim*

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Studying Evolutionary Adaptation of SARS-CoV-2

- Pp. 42-71 (30)
Samina Ejaz*, Yasir Hameed, Waqas Nazir Malik, Muhammad Usman and Uzma Karamat

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Evaluating SARS-CoV-2 Entry Pathways SARSCoV- 2 Entry Pathways

- Pp. 72-97 (26)
Muhammad Kalim*, Firasat Hussain*, Hayat Khan, Kashif Rahim, Muhammad Shuaib, Amjad Islam Aqib and Jinbiao Zhan

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Genomic Characterization of SARS-CoV-2

- Pp. 98-132 (35)
Faisal Siddique*, Muhammad Farrukh Nisar, Maida Manzoor, Firasat Hussain, Muhammad Saeed, Kashif Rahim and Asif Javaid

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Mutation of SARS-CoV-2

- Pp. 133-161 (29)
Umair Younas* and Kashif Prince

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Biochemical Characterization of SARS-CoV-2 Spike Protein Proteolytic Processing

- Pp. 162-194 (33)
Hayat Khan*, Firasat Hussain*, Muhammad Kalim, Shafi Ullah, Kashif Rahim and Faisal Siddique

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Crystal Structure Determination and Receptor Recognition Basis of SARS-CoV-2 Spike Glycoprotein

- Pp. 195-225 (31)
Maher Darwish*

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Structural Elucidation of SARS-CoV-2 Accessory Proteins

- Pp. 226-249 (24)
Tahir Shah* and Kamal Niaz

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Antigen Capture Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of the Novel SARS-CoV-2 in Humans

- Pp. 250-272 (23)
Muhammad Farooq* and Zia Ullah

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Quantification of the SARS-CoV-2 RNA in Tissues by Quantitative Real Time-PCR

- Pp. 273-300 (28)
Abhinav Anand, Neha Sharma, Sonali Bajaj, Naman Wahal, Devesh Tewari and Navneet Khurana*

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Evaluation of SARS-CoV-2 Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay

- Pp. 301-314 (14)
Shaukat Hussain Munawar*, Zahid Manzoor, Muhammad Farrukh Nisar, Muhammad Yasir Waqas and Imran Ahmad Khan

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Pseudovirus Neutralization Assay for SARS-CoV-2

- Pp. 315-336 (22)
Ankush Sharma, Sneha Joshi, Pooja Patni, Navneet Khurana and Devesh Tewari*

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Quantitative and Qualitative Determination of COVID-19 (SARS-CoV-2) Specific Antibodies Using ELISA

- Pp. 337-371 (35)
Mohammad Ejaz, Shahzad Ali and Muhammad Ali Syed*

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Chest CT Scan: An Ideal Diagnostic Tool for COVID-19

- Pp. 372-404 (33)
Noor Muhammad Khan*, Muhammad Adil, Amar Nasir and Arbab Sikandar

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Nucleic Acid-Based Detection of COVID-19

- Pp. 405-419 (15)
Abdul Basit, Ihtisham Ulhaq, Firasat Hussain, Zia-ud-Din and Kashif Rahim*

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Subject Index

- Pp. 420-428 (9)
Kamal Niaz and Muhammad Farrukh
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