Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications

by

Anupam Jyoti, Neetu Mishra

DOI: 10.2174/97898114228671190101
eISBN: 978-981-14-2286-7, 2019
ISBN: 978-981-14-2285-0



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Advances in biomedical research have had a profound effect on human health outcomes over the last century. Biophysical, biochemical an...[view complete introduction]
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Table of Contents

Foreword

- Pp. i
Rajiv Kumar
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Preface

- Pp. ii
Anupam Jyoti and Neetu Mishra
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List of Contributors

- Pp. iii
Anupam Jyoti and Neetu Mishra
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Animal Cell Culture: From Fundamental Techniques to Biomedical Applications

- Pp. 1-14 (14)
Neetu Mishra and Joyita Banerjee

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Real Time PCR as a Diagnostic Tool in Biomedical Sciences

- Pp. 15-26 (12)
Ritismita Devi, Rohit Saluja and Swapnil Sinha

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Flow Cytometry: Basics and Applications

- Pp. 27-42 (16)
Juhi Saxena and Anupam Jyoti

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Protein Structure Determination Using Experimental Phasing Technique

- Pp. 43-55 (13)
Vijay Kumar Srivastava

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Essentials of Recombinant Protein Production

- Pp. 56-72 (17)
Satyajeet Das and Sanket Kaushik

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In Silico Modeling and Drug Designing

- Pp. 73-87 (15)
Gauri Misra and Neetu Jabalia

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Biomolecular Crystallography and Its Applications

- Pp. 88-102 (15)
Nagendra Singh

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RNA Sequencing Technology for Biomedical Sciences

- Pp. 103-126 (24)
Sandeep Ameta and Roberta Menafra

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Immunoelectrophoresis: Recent Advances and Applications

- Pp. 127-137 (11)
Vinod Singh Gour and Ravneet Chug

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Subject Index

- Pp. 138-139 (2)
Anupam Jyoti and Neetu Mishra
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Foreword

Biomedical research is gaining prime attention as it is directly affecting human health. Biophysical, biochemical and cellular techniques are the backbone of biomedical sciences; a pre-requisite towards the understanding and treatment of human diseases. In the recent years, biomedical research has provided solutions for several problems faced by human beings. It is an integrated approach that includes various disciplines viz. biochemistry, microbiology, genetics and biochemical engineering. After some time, it has been integrated rapidly with new branches of science like molecular and cellular biology, genomics, proteomics, bioinformatics and nanotechnology. Biomedical research involves fundamental scientific principles applied to preclinical understanding of problems to clinical solutions. This book titled ‘Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications’, presents a broad overview regarding the basic applications of sophisticated and analytical techniques used in biophysics, biochemistry and cell biology. This book has covers the following areas: in vitro cell culture assay, real-time PCR, flow cytometry, and X-ray crystallography, discussed by authors who have quality publications in their proposed chapter area. Additionally each chapter includes application of techniques in specific areas like cells sorting by FACS, disease diagnosis by Real-time PCR, disease modeling under in vitro culture and many more. This will help students understand the importance of techniques in biophysics, biochemistry and cell biology research which will set a benchmark for further research.

As a biotechnology scientist, I am happy to recommend this book to the students of universities, both as a text and reference book. The section on technique will be used as textbook and the application section as reference. This book has been written in a way so that it is student-friendly with clean diagrams and protocols of specific techniques. I sincerely believe that the book has been prepared with the scientific skills and will serve as a useful document for the graduate and undergraduate students.

Rajiv Kumar
ICAR-Central Sheep and Wool Research Institute
Avikanagar, Tonk, Rajasthan
India


Preface

This ebook titled ‘Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications’ provides principles of basic and analytical techniques in biophysics, biochemistry and cell biology and their potential applications in biomedical research. The volume covers the basics of in-vitro cell culture techniques, such as flow cytometry including FACS, Real time PCR based disease diagnosis as well as gene expression analysis, X ray crystallography, RNA sequencing and their various biomedical applications, including drug screening, disease model, functional assays, disease diagnosis, gene expression analysis, and protein structure determination. This is a valuable resource for biomedical students, cellular and molecular biologists who want to be an eminent biomedical researcher.

We strongly believe that this book is a reader’s delight providing a comprehensive understanding on cell biology, macromolecular structure, in silico studies and functioning. It is an excellent introduction for students towards cell biology and biophysics that clearly help them to develop a wider perspective of the field. The idea to encompass knowledge covering from what is known to what is unknown in the writings from the experts in the field results in a concerted effort to justify the various topics. We sincerely hope our efforts will be embraced by students with appreciation and enthusiasm for learning.

Dr. Anupam Jyoti
Amity Institute of Biotechnology
Amity University Rajasthan
Jaipur, Rajasthan
India

Dr. Neetu Mishra
Symbiosis School of Biological Sciences (Formerly called Symbiosis School of Biomedical Sciences)
Symbiosis International (Deemed University)
Lavale, Pune, Maharashtra
India

List of Contributors

Author(s):
Anupam Jyoti


Neetu Mishra




Contributor(s):
Anupam Jyoti
Amity Institute of Biotechnology
Amity University Rajasthan, Jaipur
India


Gauri Misra
Amity Institute of Biotechnology
Amity University Uttar Pradesh, Noida
India


Juhi Saxena
Dr. B. Lal Institute of Biotechnology
6-E, Malviya Industrial Area
Jaipur 302017
India


Nagendra Singh Tomar
School of Biotechnology
Gautam Buddha University, Greater Noida, U.P.-201308
India


Neetu Mishra
Symbiosis School of Biological Sciences (Formerly called Symbiosis School of Biomedical Sciences)
Symbiosis International (Deemed University)
Lavale, Pune, Maharashtra
India


Roberta Menafra
Laboratoire de Biochimie
École supérieure de physique et de chimie industrielles de la ville de Paris [ESPCI Paris]
France


Rohit Saluja
Department of Biochemistry
Medical College Building, 3rd Floor, Saket Nagar, AIIMS Bhopal-462024
India


Sandeep Ameta
ESCPI Paris
10 rue Vauquelin, 75005 Paris
France


Sanket Kaushik
Amity Institute of Biotechnology
Amity University Rajasthan, Jaipur
India


Swapnil Sinha
Assam Downtown University
Panikhaiti, Guwahati-781026, Assam
India


Vijay Srivastava
Amity Institute of Biotechnology
Amity University Rajasthan, Jaipur
India


Vinod Singh Gour
Amity Institute of Biotechnology
Amity University Rajasthan, Jaipur
India




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