Advances in Combinatorial Chemistry & High Throughput Screening

Volume 1

by

Rathnam Chaguturu

DOI: 10.2174/97816080574501130101
eISBN: 978-1-60805-745-0, 2013
ISBN: 978-1-60805-746-7
ISSN: 2589-2835 (Print)
ISSN: 2214-515X (Online)



Indexed in: EBSCO.

Advances in Combinatorial Chemistry & High Throughput Screening, is an e-book series comprising updated research arti...[view complete introduction]
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Fluorescent Probes for Cellular Assays

- Pp. 60-83 (24)

George T. Hanson and Bonnie J. Hanson

Abstract

A fluorescent probe is a fluorophore designed to localize within a specific region of a biological specimen or to respond to a specific stimulus. Fluorescent probes have been used for nearly a century to study cellular processes due to their exquisite sensitivity and selectivity. Fluorescent probes have also gained in popularity as safety and environmental concerns over the use of radioactive probes have grown. At the same time, cellular assays are being more widely used now than ever before. This review will give a broad overview of types of fluorescent probes, types of fluorescent assays, and their application in cellular assays for a number of pharmaceutically relevant target classes.

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