Chapter 19

Non-linear Optics

Juan Carlos Stockert and Alfonso Blazquez-Castro


This chapter deals with some of the most fascinating topics in fluorescence microscopy and optics, in general: non-linear optics. All the optical phenomena explained in the previous chapters, with some particular exceptions, have dealt with linear optics; this means that a single photon is involved in the optical process during and after being absorbed. However, it is possible to have a multiphoton optical response, one that depends on more than one photon interacting with the system “at the same time”. This leads to a whole new range of optical phenomena; many of them unobservable at the relatively low light intensities with characteristic of linear optics. Some of the non-linear optical phenomena provide very interesting and useful applications in fluorescence microscopy. In this chapter, we will introduce and explain the main non-linear optical processes employed in fluorescence microscopy, enumerate their advantages and drawbacks, and highlight their importance in the life sciences.

Total Pages: 642-686 (45)

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.Mind Maps in Clinical Chemistry (Part I).
.Mind Maps in Biochemistry.
.Essential Techniques for Medical and Life Scientists: A Guide to Contemporary Methods and Current Applications- Part 2.
.Protocols used in Molecular Biology.