Chapter 6

Fluorescence Instrumental and Techniques

Juan Carlos Stockert and Alfonso Blazquez-Castro

Abstract

The use of fluorescence microscopy requires knowledge of the elements making up the fluorescence microscope. In this chapter the basic elements combining to make a fluorescence microscope will be introduced and its role explained. Afterwards an introduction to the most common fluorescence microscopic techniques will be made followed by more advanced and developed approaches. These will include total-internal reflection and time-resolved microscopies, to name a few. The chapter will end with a brief introduction to flow cytometry, a highly related fluorescence technique in the biomedical field.

Total Pages: 159-218 (60)

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