Chapter 7

Paired-End (PE), Mate Pair (MP) and Paired-End Ditag (PED) Sequencing

Kuo Ping Chiu


Information regarding the distance between paired reads enhances the accuracy of genome assembly and sequence-to-genome mapping, making paired-end indispensable strategies for DNA sequencing. The most commonly used paired-end sequencing strategies are Paired-End (PE) sequencing and Paired-End Ditag (PED) sequencing. Similarity in terminologies frequently causes confusion. This chapter is set out to clarify these terminologies and then, using PED as an example, to illustrate how a biotechnology can be sequentially developed.

Total Pages: 62-76 (15)

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