Methods: Between October 2013 and November 2013, three patients who underwent colonic stent insertion for obstructing colorectal cancer were studied. To detect viable CTCs of CRC, we employed a TelomeScanF35 detection system, which was constructed with a GFP-expressing attenuated adenovirus, in which the telomerase promoter regulates viral replication. 7.5ml of peripheral blood samples were obtained before and after stent insertion and after operation.
Results: Three patients were inserted SEMS and performed laparoscopic surgery. In one of these 3 patients, CTCs increased after stent insertion and continued in expression after the operation.
Conclusions: This study demonstrated that endoscopic stent insertion results in dissemination of tumor cells into the peripheral circulation. Although oncological consequences were not examined in this small population, viable CTC detected by TelomeScanF35 may represent a suitable prognostic marker to stratify the risk of patients with distant metastasis following stent insertion for obstructing colon cancer.
Total Pages: 80-86 (7)
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