Chapter 11

Mass Spectrometry-Based Approaches Toward Absolute Quantitative Proteomics

Keiji Kito and Takashi Ito


Mass spectrometry has served as a major tool for the discipline of proteomics to catalogue proteins in an unprecedented scale. With chemical and metabolic techniques for stable isotope labeling developed over the past decade, it is now routinely used as a method for relative quantification to provide valuable information on alteration of protein abundance in a proteome-wide scale. Recently, absolute or stoichiometric quantification of proteome is becoming feasible, in particular, with the development of strategies with isotope-labeled standards composed of concatenated peptides. On the other hand, remarkable progress has been also made in label-free quantification methods based on the number of identified peptides. Here we review these mass spectrometry-based approaches for absolute quantification of proteome and discuss their implications.

Total Pages: 261-292 (32)

Purchase Chapter  Book Details


.Sperm-Mediated Gene Transfer: Concepts and Controversies.
.Genomics in Biological Anthropology: New Challenges, New Opportunities.
.The Analysis of Regulatory DNA: Current Developments, Knowledge and Applications Uncovering Gene Regulation
.Probing Intracellular Regulation.